| Registro completo |
| Provedor de dados: |
OAK
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| País: |
Japan
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| Título: |
Construction of the recombinant pseudorabies viruses expressing Cryptosporidium parvum an immunodominant surface protein, p23
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| Autores: |
Takashima, Yasuhiro
Xuan, Xuenan
Nagasawa, Hideyuki
Matsumoto, Yasunobu
Igarashi, Ikuo
Fujisaki, Kozo
Mikami, Takeshi
Otsuka, Haruki
玄, 学南
五十嵐, 郁男
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| Data: |
2000-10
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| Ano: |
2000
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| Palavras-chave: |
Cryptosporidium parvum
P23
Herpes
Pseudorabies virus
Subunit vaccine
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| Resumo: |
To develop a vaccine against cryptosporidiosis in animals, we constructed recombinant pseudorabies virus (PrV), a member of the Herpesviridae Alphaherpesvirus subfamily, expressing an immunodominant surface protein p23 of Cryptosporidium parvum sporozoites. Because of the wide host range of PrV, it has the possibility as the vector to delivery the foreign genes to several species of animals containing experiment animal. In the recombinant constructed in this study, the p23 gene under the control of CAG promoter was integrated into the thymidine kinase (TK) gene of PRV. Antibody against p23 recognized p23 expressed in CPK cells infected with the recombinant, as the approximate 23 kDa specific band in Western blotting analysis. This study showed the possibility of a PrV recombinant as a vaccine against cryptosporidiosis in animals.
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| Idioma: |
Inglês
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| Identificador: |
http://ir.obihiro.ac.jp/dspace/handle/10322/129
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| Editor: |
National Research Center for Protozoan Diseases
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| Formato: |
application/pdf
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| Direitos: |
National Research Center for Protozoan Diseases
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